Isolation and characterization of T cells from semen
The male genital tract is of major importance in the transmission and acquisition of HIV-1. Studying cellular immunity in the male genital tract is important in development of HIV-1 vaccines protective at mucosal sites. Semen is the primary HIV-1 containing fluid released from the male genital tract and reducing virus levels in semen would also reduce HIV-1 spread. Characterizing lymphocytes from semen requires the isolation of viable T cells that can be analyzed by downstream applications such as flow cytometry. The aims of this study were to investigate the influence of various parameters on CD3(+) T cell yields from semen and to compare isolation methods to maximize CD3(+) T cell yields for the purpose of functional characterization by flow cytometry.
J. Immunol. Methods
- PMID Author:
Olivier AJ, Liebenberg LJ, Coetzee D, Williamson AL, Passmore JA, Burgers WA
Isolation and characterization of T cells from semen. J. Immunol. Methods
2012;375(1-2):223-31 doi: 10.1016/j.jim.2011.11.001