PMID: 22021856
Title: Computation guided backbone grafting of a discontinuous motif onto a protein scaffold
Abstract: The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.
Date: 1970-08-21
Year: 2011
Journal: Science
PMID Author: Azoitei ML, Correia BE, Ban YE, Carrico C, Kalyuzhniy O, Chen L, Schroeter A, Huang PS, McLellan JS, Kwong PD, Baker D, Strong RK, Schief WR
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold. Science 2011;334(6054):373-6 doi: 10.1126/science.1209368

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