A DNA-based candidate HIV vaccine delivered via in vivo electroporation induces CD4 responses toward the α4β7-binding V2 loop of HIV gp120 in healthy volunteers

Jakub Kopycinski; Hannah Cheeseman; Ambreen Ashraf; Dilbinder Gill; Peter Hayes; Drew Hannaman; Jill Gilmour; Josephine H Cox; Sandhya Vasan

doi:10.1128/CVI.00327-12

A DNA-based candidate HIV vaccine delivered via in vivo electroporation induces CD4 responses toward the α4β7-binding V2 loop of HIV gp120 in healthy volunteers

Clin Vaccine Immunol. 2012 Sep;19(9):1557-9. doi: 10.1128/CVI.00327-12. Epub 2012 Jul 25.

Authors

Jakub Kopycinski¹, Hannah Cheeseman, Ambreen Ashraf, Dilbinder Gill, Peter Hayes, Drew Hannaman, Jill Gilmour, Josephine H Cox, Sandhya Vasan

Affiliation

¹ IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom. j.kopycinski@imperial.ac.uk

Abstract

Administration of a clade C/B' candidate HIV-1 DNA vaccine, ADVAX, by in vivo electroporation (EP) was safe and more immunogenic than intramuscular administration without EP. The breadth and specificity of T-cell responses to full-length Env were mapped. Responses to multiple Env regions were induced, with most focusing on V3/C4 and V2 regions, including the α4β7 integrin-binding domain. The breadth of responses induced by this DNA vaccine regimen was comparable to that of viral-vectored vaccine regimens.

Publication types

Clinical Trial
Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
CD4-Positive T-Lymphocytes / immunology*
Electroporation / methods*
HIV Envelope Protein gp120 / genetics
HIV Envelope Protein gp120 / immunology*
HIV-1 / genetics
HIV-1 / immunology*
Healthy Volunteers
Humans
Inulin / administration & dosage
Inulin / adverse effects
Inulin / analogs & derivatives*
Inulin / immunology
Vaccination / methods
Vaccines, DNA / administration & dosage
Vaccines, DNA / adverse effects
Vaccines, DNA / immunology*

Substances

HIV Envelope Protein gp120
Vaccines, DNA
delta inulin
gp120 protein, Human immunodeficiency virus 1
Inulin