Scientific Publications
- PMID: 19715783
- Title: HIV inactivation by cross linking of photo labeled anti retroviral compounds with HIV reverse transcriptase
- Abstract: We describe a new method for the development of a preventive inactivated-HIV vaccine, based on photo-inactivation of HIV reverse transcriptase (RT), which preserves both the conformational and functional integrity of viral surface proteins. The RT of HIV-1 was selectively targeted for inactivation using a photo-labeled compound with specific affinity for HIV-1 RT. The photo-labeled virions were then exposed to UV light causing the photo-labeled compound to form a covalent bond cross-linking the photo-active compound to RT. Replication capacity of the treated virions was significantly reduced when compared to controls suggesting that exposure of treated virions to UV light had caused a stable interaction of RT and the photo-labeling compound.
- Date: 1970-08-21
- Year: 2009
- Journal: Vaccine
- PMID Author: Colby-Germinario S, Rios A, Quesada J, Anderson D, Goldstein AL, Fossum T, Wainberg MA
HIV inactivation by cross linking of photo labeled anti retroviral compounds with HIV reverse transcriptase
HIV inactivation by cross-linking of photo-labeled anti-retroviral compounds with HIV reverse transcriptase. Vaccine 2009;27(44):6137-42 doi: 10.1016/j.vaccine.2009.08.028
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