Scientific Publications
- PMID: 27013733
- Title: HIV 1 broadly neutralizing antibody precursor B cells revealed by germline targeting immunogen
- Abstract: Induction of broadly neutralizing antibodies (bnAbs) is a major HIV vaccine goal. Germline-targeting immunogens aim to initiate bnAb induction by activating bnAb germline precursor B cells. Critical unmet challenges are to determine whether bnAb precursor naïve B cells bind germline-targeting immunogens and occur at sufficient frequency in humans for reliable vaccine responses. Using deep mutational scanning and multitarget optimization, we developed a germline-targeting immunogen (eOD-GT8) for diverse VRC01-class bnAbs. We then used the immunogen to isolate VRC01-class precursor naïve B cells from HIV-uninfected donors. Frequencies of true VRC01-class precursors, their structures, and their eOD-GT8 affinities support this immunogen as a candidate human vaccine prime. These methods could be applied to germline targeting for other classes of HIV bnAbs and for Abs to other pathogens.
- Date: 1970-08-22
- Year: 2016
- Journal: Science
- PMID Author: Jardine JG, Kulp DW, Havenar-Daughton C, Sarkar A, Briney B, Sok D, Sesterhenn F, Ereño-Orbea J, Kalyuzhniy O, Deresa I, Hu X, Spencer S, Jones M, Georgeson E, Adachi Y, Kubitz M, deCamp AC, Julien JP, Wilson IA, Burton DR, Crotty S, Schief WR
- PMC Link #: PMC4872700
HIV 1 broadly neutralizing antibody precursor B cells revealed by germline targeting immunogen
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen. Science 2016;351(6280):1458-63 doi: 10.1126/science.aad9195
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Rose Catlos
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