Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer. Nat Commun 2018;9(1):3693 doi: 10.1038/s41467-018-06121-4
Scientific Publications
- PMID: 30209313
- Title: Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
- Abstract: As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans. Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design. Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers. However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity. These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.
- Date: 1970-08-21
- Year: 2018
- Journal: Nat Commun
- PMID Author: Cao L, Pauthner M, Andrabi R, Rantalainen K, Berndsen Z, Diedrich JK, Menis S, Sok D, Bastidas R, Park SR, Delahunty CM, He L, Guenaga J, Wyatt RT, Schief WR, Ward AB, Yates JR, Burton DR, Paulson JC
- IAVI Topics: HIV Immunogen Design
Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
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