PMID: 19917318
Title: Affordable in house antiretroviral drug resistance assay with good performance in non subtype B HIV 1
Abstract: The introduction of antiretroviral (ARV) therapy in resource-poor settings is effective in suppressing HIV-1 replication and prolonging life of infected individuals. This has led to a demand for affordable HIV-1 drug resistance assays, since treatment failure due to development of drug resistance is common. This study developed and evaluated an affordable 'in-house' genotyping assay to monitor HIV-1 drug resistance in Africa, particularly South Africa. An 'in-house' assay using automated RNA extraction, and subtype C specific PCR and sequencing primers was developed and successfully evaluated 396 patient samples (viral load ranges 1000-1.6 million RNA copies/ml). The 'in-house' assay was validated by comparing sequence data and drug resistance profiles from 90 patient and 10 external quality control samples to data from the ViroSeq HIV-1 Genotyping kit. The 'in-house' assay was more efficient, amplifying all 100 samples, compared to 91 samples using Viroseq. The 'in house' sequences were 99.2% homologous to the ViroSeq sequences, and identical drug resistance mutation profiles were observed in 96 samples. Furthermore, the 'in-house' assay genotyped 260 of 295 samples from seven African sites, where 47% were non-subtype C. Overall, the newly validated 'in-house' drug resistance assay is suited for use in Africa as it overcomes the obstacle of subtype diversity.
Date: 1970-08-20
Year: 2010
Journal: J. Virol. Methods
PMID Author: Wallis CL, Papathanasopoulos MA, Lakhi S, Karita E, Kamali A, Kaleebu P, Sanders E, Anzala O, Bekker LG, Stevens G, de Wit TF, Stevens W
PMC Link #: PMC2932961
IAVI Topics: HIV Assay Development

Affordable in-house antiretroviral drug resistance assay with good performance in non-subtype B HIV-1. J. Virol. Methods 2010;163(2):505-8 doi: 10.1016/j.jviromet.2009.11.011

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